ZELLX® oxidative stress testing solutions

products-servicesZellBio GmbH

June 23rd 2021

Leading life sciences research materials provider ZELLX® offers a wide range of colorimetric and fluorometric assays to quantify both enzymatic and non-enzymatic antioxidants in oxidative stress pathways.

These assays play a key role in toxicity testing and improving efficacy.

Oxidative stress

Oxidative stress is one of the most common types of toxicity. It is defined by an imbalance between the generation of reactive oxygen species (ROS) and the ability of biological systems to detoxify ROS or its related damage.

Many types of chemical, physical, and microbial agents can potentially induce oxidative-mediated stress in cells and tissues by damaging the biological components, including proteins, lipids, and DNA.

It has further been established that oxidative stress reactions can interfere with many essential biological processes such as phagocytosis (immunity and inflammation), cell respiration (mitochondria), metal metabolism, lipid synthesis, lysosomes, xenobiotic biotransformation of organic compounds and cellular signaling.

The role of antioxidants

A series of antioxidant compounds function to reduce the damaging effects of ROS. These compounds are either produced endogenously in the human body or can be obtained exogenously via nutrients.

Endogenous antioxidants are classified into two subgroups:

Enzymatics such as dismutase, reductase, catalase, peroxidase, etc.
Non-enzymatic, such as glutathione, bilirubin, metallothioneins, and uric acid.

However, as the free radical content is often higher than the capacity of endogenous antioxidants to detoxify, it is necessary to obtain some antioxidants from external sources, such as vitamin A, vitamin C, and vitamin E, to balance the disequilibrium.

ZELLX® Oxidative Stress Assays

For measuring oxidative stress, ZELLX® provides different colorimetric-based assays to evaluate the amount of both enzymatic and non-enzymatic antioxidants in oxidative stress pathways. These include:

ZX-44102-96 -Catalase (CAT) Colorimetric Activity Assay: Measure Catalase activity in any sample within 45 minutes

ZX-33102-96 – Catalase (CAT) Fluorometric Activity Assay: Measure Catalase activity in any sample within 45 minutes

ZX-44100-96 – Glutathione (GSH) Colorimetric Assay: Measure Glutathione in 20 Minutes or Kinetically from lysates, white blood cells (WB), red blood cells (RBCs), serum, plasma, urine and tissue samples

ZX-33100-96/480 – Glutathione (GSH) Fluorometric Assay: Measure Free and Total Glutathione in the same well within 30 Minutes from lysates, WB, RBCs, serum, plasma, urine and tissue samples

ZX-33104-96 – Glutathione Reductase (GSR) Fluorometric Activity Assay: Measure GSR in 20 Minutes or Kinetically from lysate, RBCs, serum or plasma

ZX-33103-96 – Glutathione S-Transferase (GST) Fluorometric Activity Assay: Measure GST Activity in 30 Minutes or Kinetically from urine, lysates, serum or plasma

ZX-44113-96 – Hemoglobin-Serum & Plasma Colorimetric Assay: Measure Hemoglobin in 30 Minutes from WB or RBC samples

ZX-44112-96 – Hemoglobin-Whole Blood & RBCs Colorimetric Assay: Measure Hemoglobin with Creatine and p-creatine in 30 Minutes from WB or RBC samples

ZX-44106-96 – Hydrogen Peroxide Colorimetric Assay: Measure Hydrogen Peroxide (H₂0₂) in 15 Minutes from fresh urine, buffer, or TCM samples

ZX-33106-96 – Hydrogen Peroxide Fluorometric Assay: Measure Hydrogen Peroxide (H₂0₂) in 15 Minutes from fresh urine, buffer, or TCM samples

ZELLX® ZX-44107-96/192 – Nitric Oxide (NO) Colorimetric Assay Kit: Measures Nitrite (NO₂), Nitrate (NO₃) and Nitric Oxide (NO) within 5 minutes in a variety of samples, including Water, Serum, Plasma, Urine, Saliva, Buffers and TCM

ZX-44108-96/192 – Superoxide Dismutase (SOD) Activity Colorimetric Assay: Measure SOD Activity from serum, plasma, cell, tissue or Erythrocyte Lysates samples

ZX-44109-96/192 – Total Antioxidant Capacity (FRAP) Colorimetric Assay: (Colorimetric): Measure Ferric Reducing Antioxidant Power (FRAP) in 30 Minutes from serum, plasma, urine, cell lysate, food, cosmetic, and other samples

ZX-55100-96 – 8‐OHdG (DNA Damage) ELISA Kit: Measure oxidized derivative of deoxyguanosine (8-OHdG) molecules formed during the repair of damaged DNA by exonucleases in 2.5 Hours from serum, plasma, saliva, urine, fecal extracts, or TCM samples.