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ZELLX® urine and serum creatinine assay kits
Measuring levels of Creatinine (2‐amino‐1‐methyl‐5H‐imidazol‐4‐one) in urine or serum is a key test for diagnosing conditions such as diabetes and cardiovascular disease. It is vital that such assays correspond to the standard reference curves defined by the US-based National Institute of Standards and Technology (NIST) or UK-based National Institute for Biological Standards and Control (NIBSC).
ZELLX® offers colorimetric assay kits for measuring creatine levels in urine or serum that are calibrated to NIST standards. These kits are also species independent and so can be used for creatinine measurements in humans and a range of other mammals.
ZELLX® creatinine assay kit benefits
Both the urine and serum creatinine assays use high quality Creatinine Standard solutions calibrated to NIST defined standards for precise and referenced creatinine measurement.
These are high sensitivity assays that can detect to levels of 0.19 mg/L in urine or 0.81 mg/L in serum, with full species independence for measuring creatinine levels in human and other species.
Other benefits include stable long term storage at 4°C, competitive pricing, assured high quality standard and ability to measure creatinine in serum and urine separately.
Using the ZELLX® Creatinine Assays
The kits include 200 μL of ZELLX® Creatinine Standard calibrated to NIST standard, allowing the assay to generate a standard curve with all samples read off the standard 1000 mg/L curve. The kits also include 10 mL of ZELLX® Creatinine Reagent, and a Clear Half Area 96 Well Plate.
A creatinine standard, standards or diluted samples are pipetted into the clear microtiter plate, with assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the Creatinine Reagent, which is pipetted into each well. The Jaffe reactions used in the kits are optimized to reading of creatinine levels in urine or serum respectively.