ZELLX® Corticosterone ELISA & CLIA assay kit

products-servicesZellBio GmbH

July 13th 2021

ZELLX® has developed a Corticosterone Assay Kit that can precisely quantify corticosterone levels in sample sizes as small as 2μL within 90 minutes.

The Corticosterone Assay Kit is available in 96-well ELISA and CLIA versions and can quantify corticosterone from a wide range of sample types, including serum, plasma, urine, saliva, dried fecal extracts, and TCM.

Corticosterone measurement

Required sample size is a critical issue in biomedical studies, with ability to measure analyte in smaller sample quantities leveraging ability to carry out more analyses in lower time.

The ZELLX® Corticosterone Assay series has been developed based on chemiluminescent immunoassay (CLIA) and on the competitive ELISA method which is ideal for quantification of small antigens even at low concentrations.

Furthermore, these assays are species independent and provide precise corticosterone measurements across three different ranges:

Corticosterone CLIA: 6.7-3000 pg/mL
Corticosterone ELISA: 20.9-10000 pg/mL
Corticosterone high sensitivity ELISA: 14.35-5000 pg/mL

This versatility allows the assay series to cover the full range of biological study needs.

User advantages

The ZELLX® Corticosterone Assay series deliver a clear set of tangible user advantages for research teams and laboratories:

Competitive pricing Low price
Very high sensitivities
Choice of measurement ranges
Low sample size requirements

Research interest

Corticosterone (C21H30O4, Kendall’s Compound ‘B’) is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is secreted in response to stimulation of the adrenal cortex by Adrenocorticotropic hormone (ACTH), and is the precursor of aldosterone.

Along with Cortisol, Corticosterone is one of the end products of the complex series of direct interactions and regulations along the hypothalamic–pituitary–adrenal axis (HPA axis), which plays an important role in the stress response. The HPA axis regulates many other biological processes, including digestion and energy usage, the immune response, sexuality and emotions.

Stress-induced activation of HPA axis up-regulates the plasma level of glucocorticoid hormones (cortisol in human, corticosterone in rats and mice). Cortisol, as a steroid hormone, regulates a wide range of biological processes including the immune response, metabolism, and most importantly it plays a key role in stress response. 11β-hydroxysteroid dehydrogenase type 2 (11HSD2) on the other hand deactivate the cortisol by oxidizing it to cortisone, and prohibits receptor activation. Corticosterone despite its presence has a minor importance in human, however, in rodents, birds and reptiles, corticosterone is the primary glucocorticoid.

Corticosterone is therefore a major indicator of stress in nonhuman mammals.

Studies have shown a link between corticosterone‐mediated increased level of stress and impairment of long term memory retrieval, as well as chronic corticosterone elevation due to dietary restrictions and in response to burn injuries. In addition to stress levels, corticosterone has been indicated as playing a decisive role in sleep‐wake patterns.