ZELLX® colorimetric nitrous oxide NO assay kit

products-servicesZellBio GmbH
June 9th 2021

As a free radical and signalling molecule, Nitric oxide (NO) plays an important role in vasodilation, immune system function, apoptosis and many other physiological and pathological processes. Nitric oxide is generated either via the oxidation of L-arginine by NO synthases (NOSs) or the reduction of dietary Nitrite (NO2) and Nitrate (NO3) by various metal-containing proteins.

Nitric oxide is an unstable molecule that quickly degrades, which makes it challenging for direct detection methods. However, ZELLX® has now developed an assay kit that exploits NO2 and NO3 characteristics as the stable metabolites of NO oxidation, allowing their concentrations to be conveniently measured via colorimetric methods and reported as the total NO content in biological samples.

ZELLX® ZX-44107-96/192 Nitric Oxide (NO) Assay Kit (Colorimetric)

The ZELLX® Nitric Oxide Assay Kit measures Nitrite (NO2), Nitrate (NO3) and Nitric Oxide (NO) in a variety of samples with accurate quantification achieved using the standard curve method, achieving standard reference quality, which is the most critical part for any bioassay.

The assay uses the Standard calibrated to The National Institute of Standards and Technology (NIST).

ZELLX® Nitric Oxide Assay Kit benefits

Apart from its distinctive status as an NIST standard calibrated Nitric Oxide assay kit; this ZELLX® colorimetric assay kit presents users with a range of tangible benefits. These include:

  • Low price
  • high quality
  • reliable standard
  • high sensitivity (2.63 µM for Nitrite & 1.02 µM for total NO)
  • fast results (Measure Nitrite in five minutes, Total NO in 30 minutes)


Click on ZX-44107-96/192 | Nitric Oxide (NO) Assay (Colorimetric) for product details.

ZELLX® colorimetric nitrous oxide NO assay kit

Typical standard curve of ZELLX® NO Assay kit

ZELLX® colorimetric nitrous oxide NO assay kit