Sharing advanced modelling methods at HPLC 2025 in Bruges

Berlin: – The MOLNÁR-INSTITUTE for Applied Chromatography is proud to announce its participation as a Bronze Sponsor, scientific contributor, and exhibitor at the upcoming International Symposium on High Performance Liquid Phase Separations (HPLC 2025).  Taking place in Bruges, Belgium, HPLC 2025 will feature cutting-edge research presentations, technical workshops, and exhibitions from global leaders in the industry.

The DryLab® MOLNÁR-INSTITUTE stand at Booth 14 in the Exhibition Zone of the BMCC Bruges Conference Center will present all the new developments – updated modules and features of the DryLab®4 separation modelling software. For over 40 years, DryLab® has supported method development in modern high-performance liquid chromatography (HPLC), contributing to improved efficiency and reliability in life science applications across pharmaceutical, food, and environmental analysis.

With its strong focus on innovation in cutting edge industry solutions, laboratory automation, and data-driven analytical techniques, HPLC 2025 provides an essential platform for advancing the science of separation and enhancing laboratory performance – areas where The MOLNÁR-INSTITUTE continues to lead and innovate.

Learn how to work smarter in chromatography! Visit the MOLNÁR-INSTITUTE booth for live demonstrations, join cutting-edge presentations, and meet the team behind one of the industry’s leading method development platforms.

Stationary Phase Selection in MS-analysis of Oxysterols

As part of its commitment to advancing analytical technology, the DryLab® scientific team will present two contributions highlighting model-assisted applications in chromatographic method development.

The first study, led by Andrea Castellaneta from Aldo Moro University of Bari, Italy, is titled “Tackling the Challenge of Oxysterols Separation by High-Performance Liquid Chromatography: The Application of Analytical Design Space Modelling to Explore the Capabilities of Different Stationary Phases.” This work was conducted in collaboration with Dr. Imre Molnár, President and Dr. Hans-Jürgen Rieger, Vice President of the MOLNÁR-INSTITUTE.

The study demonstrates the successful use of separation modelling to enhance the robustness of hyphenated LC-MS methods for the analysis of oxysterol compounds. Using DryLab® software, the chromatographic separation was optimized across C18 and cyanopropyl stationary phases, resulting in a robust method without the need for derivatization agents – a significant improvement over previous approaches. Overall, these results have contributed to the development of a more reliable RPLC-FTMS method, offering a robust and efficient platform with minimal sample preparation requirements.

Tackling pH challenges in RPC

The second scientific contribution to the symposium will be a poster summarizing a recent study published in the Journal of Chromatography A, titled “Extended Multidimensional Design Space Studies of Volatile and Non-Volatile Buffer Systems.” The work was led by Arnold Zöldhegyi, Senior Scientist at the Molnár-Institute, in collaboration with Dr. Imre Molnár and other prominent researchers, Dr. Krisztián Horváht from the University of Pannonia and Dr. Róbert Kormány from Egis Pharmaceuticals Plc. in Hungary.

The study addresses the challenges of chromatographic selectivity and the choice of pH modifiers in the separation of ionizable compounds by HPLC. Using DryLab®4 modeling within an Analytical Quality by Design (AQbD) framework, the team developed and compared three-dimensional (tG-T-pH) separation models for terazosin and selected impurities across a pH range established using both volatile and non-volatile buffers.

The results revealed not only expected equivalences within overlapping Method Operable Design Regions (MODRs), but also highlighted buffer-specific selectivity differences with significant implications for method robustness. This study underscores the critical role of buffer selection in developing reliable, high-performance separation methods.

Don’t miss the chance to connect, learn, and be inspired — see you in Bruges!

About The MOLNÁR-INSTITUTE

Founded in 1981, The MOLNÁR-INSTITUTE develops DryLab®4, a software for UHPLC modelling for a world-wide market. Its powerful modules gradient editor, peak tracking, automation, robustness and Design Space Comparison allow for the most sophisticated method development as required across modern pharma industries. Analytical scientists use DryLab®4 to understand chromatographic interactions, to reduce analysis time, to increase robustness, and to conform to Analytical Quality by Design (AQbD) principles, according to the recently published ICH Q14 regulatory framework.

The MOLNÁR-INSTITUTE is a registered partner of the US-FDA, CDC and many other regulatory bodies. DryLab®4 pioneered AQbD long before regulatory agencies across the world encouraged such submissions. Widely implemented by thought leaders, the software contributes substantially to the paradigm shift towards a science and risk driven perspective on HPLC Quality Control and Assurance.

Further information at: http://www.molnar-institute.com/

About HPLC Symposium 2025

The International Symposium on High Performance Liquid Phase Separations (HPLC) is an international scientific forum focusing on high-performance liquid chromatography in its various forms, along with complementary separation techniques. These include electrophoresis, electrochromatography, field-flow fractionation, supercritical fluid chromatography and hyphenated techniques, such as LC/MS, CE/MS and SFC/MS.

The symposium also considers microfluidic, nanofluidic and chip separations, diagnostic systems, and other leading technologies.

Founded in 1973, the annual symposium alternates between venues in Europe and the USA. Following the previous 2024 conference held in Colorado, USA, the 52^nd HPLC Symposium will return to Europe as a five-day event opening June 15 at the ultra-modern BMCC Conference Center in the center of the medieval Flanders city of Bruges. The 2025 Symposium will focus on industrial research and the automation of the analytical lab and its data flows.

The 2024 event is organized by Conference Catalysts LLC with further information at: https://hplc2024-symposium.org/about/conference-history

Resources

Click on Molnár-Institute Method Development Services for other information.