Molnár-Institute founder authors guide to computer-aided HPLC chromatography

Berlin: – Imre Molnár, founder and President of The Molnár-Institute for Applied Chromatography, has teamed up with another world-acknowledged expert, Szabolcs Fekete, to produce a new author handbook guide to leading edge techniques in high performance liquid chromatography (HPLC).

The Book ‘Software-Assisted Method Development in High Performance Liquid Chromatography’ is jointly edited by Dr. Molnár and Dr. Fekete, of University of Geneva, and aims to provide a general overview of the new possibilities opening up in HPLC method development, due to recent developments in chromatographic retention modeling.

Models explained

It presents many of these current possibilities in software assisted liquid chromatographic method modeling, including Reversed-Phase Liquid Chromatography (RPLC), Ion Exchange Chromatography (IEX), Hydrophobic Interaction Chromatography (HIC), and Hydrophilic Interaction Liquid Chromatography (HILIC) in detail. It exposes the most important variables for tuning retention and selectivity for all these chromatographic modes.

Beside the industrial and practical benefits of retention modeling, it also illustrates the possibilities in teaching and education.

Practical guide

Their book, published by World Scientific, is aimed at practicing chromatographers looking to save time, costs and effort in method development and looking for a deeper understanding of the retention behavior of their samples. It shows how computer-aided method development is also useful for studying conditions for method robustness.

The same technologies can also revolutionize transfer and scale of methods and automate the method development procedure.

Real world examples

The book reviews the most important chromatographic modeling software packages currently available and presents step-by-step screening and optimization procedures for various samples and modes of separation.

Several industrial examples illustrate the potential and benefits of computer-assisted method development from various fields, highlighting the benefits, time and cost savings offered by state-of-the-art software assisted HPLC method development.

About Molnar-Institute

Molnár-Institute for applied chromatography focuses on new strategies for improving the efficiency and success of modern high-performance liquid chromatography (HPLC) through its method development software DryLab®4, HPLC and software training courses, and HPLC consulting.

Since its foundation in 1981, by Dr. Imre Molnár in Berlin, Molnár-Institute has followed a consistent mission, to form a center of excellence to save, reduce and optimize method development and run time in HPLC.

The institute supports ongoing research and development at universities and corporations worldwide with a team of chromatography specialists devoted to improving global health care and to ensuring the development of safe and effective products in the pharmaceutical, life-science, and food industries.

By applying its proprietary method development software DryLab®4 in conjunction with rigorous methodologies, the institute is able to optimize method development and reduce method run time in HPLC and UHPLC. Using real data from a minimal number of experimental runs, DryLab® builds multi-dimensional selectivity maps depicting the interaction of the most important parameters influencing an HPLC separation. This systematic, guided method development process can halve development time, help users optimize parameters based on their specific method goals, and enable easier compliance with regulatory standards (QbD).

Dr. Imre Molnár remains president of the Molnar-Institute, which is a privately-owned organisation, headquartered in central Berlin.