High level methanol-free phytase production in Pichia pastoris
WHITE PAPER | VTU Technology GmbH
MARCH 15, 2017
Harnessing VTU Technology’s Pichia pastoris protein production toolbox and screening know-how in a case study, the production of an engineered variant of Butiauxella sp. phytase yielded 22 g/L of enzyme in a methanol-induced process and 20 g/L under methanol-free conditions, constituting the highest amounts of yeast-produced recombinant phytase reported so far.
This case study reveals that the appealing features of PAOX1 driven expression such as tight regulation of the production process while facilitating high-level protein production are equally effective with VTU Technology´s MeOH-free AOX1 promoter variants.
Unlock Pichia by increasing genetic diversity
The results presented in this new white paper clearly underline the usefulness of VTU´s AOX1 promoter library and the company´s entire expression toolbox as well as strain development and cultivation protocols to unlock Pichia as a powerful host for recombinant protein production. Using our approach, we could demonstrate that increasing genetic diversity leads to a significant improvement of product yields.
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