Does acidity matter? The MOLNÁR-INSTITUTE to address pH issues in HPLC at EAS 2024

Berlin: – The MOLNÁR-INSTITUTE for Applied Chromatography (Molnár) is returning to the Eastern Analytical Symposium (EAS) at Plainsboro, New Jersey, USA, as an exhibitor and contributor to the scientific program.

Senior Scientist at The MOLNÁR-INSTITUTE, Arnold Zöldhegyi, will deliver a technical presentation to the session on Leveraging Modeling for Chromatographic Analysis, on the afternoon of the final day of the conference.

New DryLab Modeling Application

As an exhibitor at the Symposium, The MOLNÁR-INSTITUTE ’s customary stand at Booth L3 at the Crowne Plaza Princeton Conference Center will showcase latest developments of its DryLab®4 software modelling and analytical platform that is revolutionizing the world of high-performance liquid chromatography (HPLC).

DryLab capabilities also feature in the Arnold Zöldhegyi’s presentation ‘Does pH Matter? Comparing Multidimensional Design Spaces of Volatile and Non-Volatile Buffer Systems’ (November 20, 14:10pm), summarizing a study he carried out in collaboration with Institute President Dr. Imre Molnár, Dr. Krisztián Horvath, of University of Pannonia, and Dr. Robert Kormány of Egis Plc in Budapest.

The study sheds new light on puzzling questions about which pH-modifiers to use for separating ionizable compounds in high performance liquid chromatography. This is particularly so for reversed-phase (RP) applications, where defining pH proves challenging due to the continuous presence of organic solvent. Temperature variations and varying buffering capabilities further complicate acidity and alkalinity relationships.

Surprising Results

The study team used The MOLNÁR-INSTITUTE ’s analytical software platform DryLab4 and AQbD-based modeling methodology to discover whether a volatile acetate buffer could replace an equimolar non-volatile phosphate buffer within the equivalent pH range.

After comparing separation models of terazosin and selected impurities constructed as 3-D models, the team was surprised to identify not only expected equivalences in the shared Method Operable Design Regions (MODRs), but also clear differences between the two buffer systems.

About The MOLNÁR-INSTITUTE

Founded in 1981, the MOLNÁR-INSTITUTE develops DryLab®4, a software for UHPLC modelling for a world-wide market. Its powerful modules gradient editor, peak tracking, automation, robustness, and Design Space Comparison allow for the most sophisticated method development as required across modern pharma industries. Analytical scientists use DryLab®4 to understand chromatographic interactions, to reduce analysis time, to increase robustness, and to conform to Analytical Quality by Design (AQbD) principles, according to the recently published ICH Q14 regulatory framework. DryLab contributes strongly to the “green chromatography movement”, as a model chromatogram is produced in one second, allowing great saving of organic eluents.

The MOLNÁR-INSTITUTE is a registered partner of the US-FDA, CDC, and many other regulatory bodies. DryLab®4 pioneered AQbD long before regulatory agencies across the world encouraged such submissions. Widely implemented by thought leaders, the software contributes substantially to the paradigm shift towards a science and risk driven perspective on HPLC Quality Control and Assurance.

Further information at: http://www.molnar-institute.com/

About EAS 2024

The Eastern Analytical Symposium and Exposition (EAS) is held each year to provide professional scientists and students working in the Eastern USA with continuing education in the analytical and allied sciences through the presentation of symposia of papers, workshops, and short courses.

For more than 50 years, the EAS Technical Program has provided cutting-edge information for the analyst with research presentations on practical and novel technologies. The symposium now typically contains over 450 papers on a wide variety of subjects, from pharmaceutical analysis to art conservation, emphasizing practical applications of a wide range of technologies including Liquid chromatography, Raman spectroscopy, microscopy, and sensors in solving real-life problems.

The 2024 EAS Symposium will again be a three-day event, opening November 18 at the Crowne Plaza Princeton Conference Center in Plainsboro, NJ. The 2024 conference will be themed ‘Partners in Problem Solving’ reflecting its focus on how analytical scientists are uniquely positioned to build strong cross-functional partnerships and enable complex solutions through leveraged data.

The conference will include technical sessions, short courses, special lectures, workshops and student seminars, as well as a co-located exhibition of analytical science apparatus and supplies.

The event is organised by EAS with further information available at: https://eas.org

Resources

Click on DryLab® – Software for Analytical Design Space Modeling for further information