PHCbi IncuSafe incubation for secure cell cultures

Combining advanced technology, unique design features and high-quality engineering, PHCbi IncuSafe CO₂ and Multigas Incubators create precise and regulated environments for cell culture.

Providing outstanding performance and flexibility, this innovative range of incubators enables users to replicate in-vivo conditions very closely for optimal results and reproducibility.

IncuSafe Advantages

IncuSafe incubators deliver three key benefits:

• Precisely regulated environment: IncuSafe Incubators offer accurate, uniform and highly responsive control of conditions within the chamber. Temperature is regulated through three independent heating zones under microprocessor PID control. High quality sensors within the incubators ensure excellent control of CO₂ and O₂.
• Active background decontamination: IncuSafe Incubators are designed to actively prevent contamination during cell culture. The unique, copper-enriched stainless steel alloy interior eliminates contamination and mitigates the effect of airborne contaminates that can be introduced through normal use. An optional, isolated, UV lamp decontaminates circulating air and water in the humidifying pan, without harming cultured cells.
• Versatile sterilization regimes: PHCbi IncuSafe offers users a choice of sterilization methods. For fast turnaround, H₂O₂ decontamination safely cleans the chamber in less than three hours. Another option is heat sterilization to complement background decontamination. PHCbi’s Dual Heat Sterilization (available in the MCO-170AICD CO₂ Incubator) provides an 11-hour, 180°C sterilization process. With extremely low heat dissipation, cell culturing can continue uninterrupted in stacked IncuSafe Incubators during sterilization.

IncuSafe Technologies

IncuSafe Incubators deliver user benefits thanks to a range of distinctive or proprietary technologies:

Direct Heat System: The direct heat system achieves an accurate, uniform, and highly responsive temperature control within the chamber. It regulates temperature through three independent heating zones under microprocessor proportional–integral–derivative (PID) control. This control loop feedback mechanism continuously calculates an error value as the difference between desired setpoint (SP) and measured process variable (PV) and corrects using the three PID terms.

CO₂ control by IR Sensor: The infra-red based system offers accurate and reliable CO₂ measurement using a ceramic-based sensor that is unaffected by moderate changes in temperature and relative humidity and is linked to the PID controller for fast recovery times.

Dual IR Sensor: The single beam, dual detector IR CO₂ sensor simultaneously measures sample and reference wavelengths for continuous auto- zero calibration. It thus provides continuous calibration for unprecedented control, accuracy and stability.

Zirconia O₂ Sensor: The unique, solid state Zirconia O₂ sensor delivers precise oxygen control. (1-18%; 22-80%), for physiological oxygen levels that simulate in vivo conditions.

InCu-saFe Germicidal Interior: The inCu-saFe copper-enriched stainless steel alloy interior combines the germicidal properties of copper with the corrosion resistance of stainless steel.

SafeCell UV Lamp: The optional, isolated, SafeCell UV lamp decontaminates circulating air and water in the humidifying pan, without harming cultured cells.

H₂O₂ Decontamination: The unique H₂O₂ decontamination system delivers fast and validated decontamination. The system uses vaporized H₂O₂ for a minimum 6 log reduction in major contaminants and full decontamination in under three hours.

Dual Heat Sterilization: Dual heat sterilization utilizes the incubator’s two heaters during the 180°C sterilization process, which takes 11 hours. There is no effect on temperature inside stacked incubators due to low heat dissipation so cell culturing can continue uninterrupted with no need to remove inner parts like the CO₂ sensor and UV light, or recalibrate after sterilization. This allows more efficient laboratory routines with less incubator downtime. The dry heat sterilization cycle is controlled through the incubator microprocessor control system. The outer door is locked automatically upon initiation of the sterilization cycle and unlocked upon completion.

The IncuSafe Incubator range

PHCbi IncuSafe incubator cabinets are available in a wide range of sizes and formats, from 50 liter capacity all the way up to 850 liters.

50 litre cabinets

MCO-5AC-PE IncuSafe CO₂ Incubator: This compact CO₂ Incubator provides an excellent small scale solution for individual cell culture, optimized for patient and/or cell line specific cultures. The copper-enriched stainless steel alloy prevents contamination during cell culture while improved temperature stability with PHCbi’s proprietary DHA (Direct Heat and Air jacket) System and PID-controlled three-way heaters provide a high-precision temperature environment, minimal risk of condensation and shortest recovery times for set parameters. When arranged in triple stackable configurations, this unit meets GLP requirements by helping to avoid patient-to-patient cross contamination in individualized patient-specific clinical incubation applications.

MCO-5M-PE IncuSafe Multigas Incubator: This personal-size compact Multigas Incubator features a similar triple stackable format with variable CO₂ and O₂ control to simulate in vivo conditions, making it ideally suited to one-donor/one-chamber use to incubate patient cells, in-vitro fertilization, and for dedicated research project use in laboratories. The inCu-saFe germicidal interior and SafeCell UV lamp combine to continuously prevent contamination during cell culturing.

170 litre cabinets

MCO-170AIC Series CO₂ Incubators: The IncuSafe MCO-170AIC and MCO-170AICD CO₂ Incubators provide precise control of CO₂ concentration and accurate, uniform, and highly responsive temperature control within the chamber. During cell culturing the inCu-saFe germicidal interior and SafeCell UV lamp work continuously to prevent contamination. Both feature data logging, LCD touchscreen display and USB connectivity. The MCO-170AIC features optional H₂O₂ Decontamination while MCO-170AICD features 180C Dual Dry heat sterilization.

MCO-170M IncuSafe Multigas Incubators: The MCO-170M series helps to achieve more accurate results when culturing cells at physiological oxygen levels that provide a highly stable virtual in-vivo environment for highly reproducible cell culturing. Dual IR sensor provides precise regulation of CO₂ levels while solid state Zirconia sensor ensures precise regulation of O₂. The MCO-170M multi gas incubator is ideal for cell culturing under hypoxic conditions and is therefore ideally suited for mammalian tissue research applications, cellular therapy, stem cell research, pathophysiological studies and pharmaceutical production processes. These incubators are available in -PE standard model, UV (featuring SafeCell ultra-violet lamp) and UVH variant with additional H₂0₂ decontamination.

230 litre format

MCO-230AIC IncuSafe CO₂ incubator: The IncuSafe MCO-230AIC CO₂ incubator incorporates similar features to the MCO-170AIC Series to provide precise control of CO₂ concentration and accurate, uniform, and highly responsive temperature control within the chamber, along with inCu-saFe germicidal interior and SafeCell UV lamp work for continuous contamination control. However, the increased 230 litre capacity provides opportunities to use the incubator to scale up from R&D to clinical trials and manufacturing.

850 litre format

MCO-80IC IncuSafe CO₂ incubator: This large-scale cell culture CO₂ Incubator incubator with its reach-in shelved format is ideal for culturing high volumes of samples with optimum temperature and CO₂ uniformity throughout the chamber and rapid recovery after door openings with exceptionally low CO₂ gas consumption. The full view, double paned glass door allows easy observation of culture while the door heating system combined with specially designed air flow system prevents condensation throughout the cabinet.

Resources

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