Online Peptide Guide

Leading CDMO Bachem AG now hosts an online guide to peptides on its website (see Resources for link).

The Online Peptide Guide for Beginners was created by Bachem, drawing on its unrivalled knowledge and manufacturing expertise in small molecule substances, including peptides, oligonucleotides, and proteins. The guide is intended to provide students, researchers, and partners with all the basic information needed by anyone working with peptides.

Key topics

The peptide guide is structured into eight chapters, each covering an essential aspect of peptide technology:

• Peptides & Amino Acids for Beginners: This section answers the basic questions of what peptides are, their importance to medical science, their relationship to amino acids, and the basic structures and notations involved.
• Peptide Manufacturing: A simplified guide to how peptides are manufactured, including the key steps involved in chemical synthesis, and how to protect against amino acid side reactions.
• Peptide Synthesis Methods: The four basic methods for synthesizing peptides explained: Solid-Phase Peptide Synthesis (SPPS), Liquid-Phase Peptide Synthesis (LPPS), Molecular Hiving™, and Chemo-enzymatic Peptide Synthesis (CEPS). The chapter also explains how Bachem employs these techniques, combining traditional methods with innovative approaches.
• Peptide Purification: Outlining the methods and steps involved in impurity removal, standard RP-HPLC purification, and alternatives for challenging peptides. This section also addresses scalability using methods like CCD, the impact of basic groups on salt formation, and introduces MCSGP technology that offers higher yields and improved sustainability.
• Quality Control: Focusing on the QC processes for peptide products post-purification and lyophilization, this chapter covers methods to confirm product identity, purity, and content, including techniques like TLC, HPLC, and mass spectrometry. It also addresses batch variability, handling of residual contaminants, and recommended purity levels for different applications.
• Peptide Modification: Explaining how peptides can be enhanced for stability, functionality, and biological activity, through N-terminal, side chain, and C-terminal modifications.
• Handling And Storage: A review of best practices for handling peptides, including how to maintain their stability during storage, proper techniques for solubilization, and precautions needed for sensitive peptide types.
• Glossary: A comprehensive list of the acronyms and abbreviations encountered in the world of peptides, covering analytical methods, chemical compounds and key terminology relevant to peptide synthesis and analysis.

The guide makes clear how peptides are at the heart of cutting-edge research and innovative therapies, from NCE development to generics. It also shows the different ways in which Bachem can support advancies in peptide science and therapeutic development.

The Online Peptide Guide for Beginners forms part of a suite of Bachem scientific tools, including a Peptide Calculator, Peptide Content Calculator, Oligo Calculator, and a series of peptide-related blogs, webinars, and technical papers.

Resources

Click on Bachem Guide: Peptides for Beginners to explore the online guide.
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