Prokaryotic Protein Expression

enGenes Biotech has developed the prokaryotic protein expression system enGenes-e^Xpress™ as a leading edge microbial protein production system for optimized protein expression.

Growth decoupled expression of proteins in prokaryotes such as Escherichia coli (E. coli) allows enGenes to develop economic, scalable and efficient production processes within short time frames to make unique biotech products commercially viable.

Advantages of enGenes-e^Xpress prokaryotic protein expression

enGenes-e^Xpress offers a modular platform for gene expression and protein production in E. coli and will be expanded to other prokaryotes, notably Bacillus subtilis, in the near future. This forms the basis for a similarly modular suite of enGenes services focused on the expression of difficult-to-express proteins.

The service menu is divided into a series of steps which can be performed either individually or in combination – with customers able to select which steps they actually need. The modular approach allows more scalable and cost-effective manufacturing processes for recombinant proteins.

Thus the enGenes-e^Xpress technology for growth-decoupled protein production forms a next-generation E. coli expression platform that allows for scalable production of difficult-to-express or even “toxic” recombinant proteins at a fraction of their current cost.

enGenes prokaryotic protein expression services

Based on enGenes-e^Xpress technology and method portfolio, enGenes can deliver improved host cell lines, plasmid expression vectors and optimized production processes to maximize bioprocess efficacy.

Customized project plans also responds to individual documentation requirements to support easy technology transfer to clients’ own facilities and quality management systems.

enGenes-e^Xpress forms the key building block in a modular system of customer services for gene expression and optimized protein production in E. coli, Bacillus subtilis and other prokaryotes.

These prokaryotic protein expression services, focused on difficult-to-express proteins, are offered as a series of options or ‘steps’ covering all specific demands along the line from gene to protein.

These steps, which can be performed individually or in combination, comprise:

• Cloning / Vector optimization
• Generation of expression strain and host cell engineering
• Feasibility studies for enGenes-e^Xpress technology
• Upstream- and Downstream process development
• Production of non-GMP material for research in mg- to g-scale

Along with the regular project updates, clients also receive step-by-step progress reports with detailed documentation to comply with their internal quality systems.