Precision Protein Engineering using Non-canonical Amino Acid Incorporation

This whitepaper introduces the enGenes-e^xpand technology, the site-specific incorporation of non-canonical amino acids (NCAAs) by amber-stop codon suppression. This technology has vast applications in fields like protein engineering, drug discovery, and biotechnology, by enabling the synthesis of modified proteins with tailored properties, as well as the cost-effective incorporation of NCAAs into proteins for bioconjugation through click-chemistry. This approach, which overlaps with biorthogonal chemistry, offers specific, sensitive, rapid, and easy-to-handle methods for protein modification under physiological conditions. Click chemistry has been applied in diverse areas such as imaging, labelling, sensing, drug design, and enzyme technology, highlighting its potential in protein engineering and medical industries.