Fragment Library Screening

ZoBio offers fragment library screening; we can provide our own highly validated fragment library or can work with a customer’s library to provide ligand screening using ZoBio’s proprietary TINS technology.

• TINS uses a single sample of the target and a reference protein that have been immobilized on sepharose based resin.
• Fragments to be assayed for binding are injected as mixtures of between 3 and 9 compounds.
• Binding is detected by a reduction in the height of the NMR signals of a compound that specifically binds to the target.
• Comparison of the NMR spectra of the fragments in the presence of the target and reference proteins directly reveals the identity of a ligand.

TINS is a powerful approach to hit discovery that has been validated on a wide range of targets including viral proteins, proteases, kinases, protein-protein interaction targets, molecular chaperones and membrane proteins such as GPCRs.

A typical TINS hit discovery and hit characterization/validation project is completed in 1-2 months.

For more information or to discuss fragment library screening please contact us directly.