Combined pFlexMAM Protein display system – tANCHOR

Overview

The latest White Paper from German-based synthetic biology, combinatorial gene design and multi-gene expression specialists ATG: biosynthetics describes how its FlexTEC mammalian expression system for trans-membrane transport of epitope and protein entities can be fused with tANCHOR protein to create the pFlexMAM protein display system. This allows fusion proteins to be predominantly displayed on the surface of mammalian (e.g. human) cells.

tANCHOR is the FlexMAM family member of vectors intended for hitchhiker fusion protein membrane transport translocations as well as anchored protein display on the cellular surfaces.

The paper shows how the combination of tANCHOR with FlexTEC Expression Technology Platform can provide ways for synthetic genes to express one to multiple genetic units in one construct.

Abstract Summary

The White Paper describes the mechanism of action of trans-membrane transport of epitope and protein entities fused with the tANCHOR protein, showing how fusions proteins created this way, such as tANCHOR-mCherry fluorescence reporter protein are predominantly displayed on the cell surface.

It shows potential application of tANCHOR for the systemic immunization by vaccines, in which epitopes are presented on the surface of a carrier protein inducing the production of antibodies which are directed against the epitopes displayed and how use of tANCHOR can allow display of transfected complete autologous cells or just its membrane fractions.

It also describes a direct DNA – immunization approach in which tANCHOR vector is tranfected into muscle tissue via a GeneGun as an example of personalized medicine approaches preventing aseptic inflammatory reactions.

A further example of extraordinary interest is using tANCHOR directly for antibody testing where the relevant epitopes are exposed directly on the cell surface; an experimental setup that can be performed in a 96-well liquid handling format with cells seeded, grown and transiently transfected with antigenic determinants like antigens and epitopes encoded by synthetic gene constructs.

Figures in the White Paper illustrate basic mechanisms of action, the range of translocation, selection, transfer and maintenance combinations, the range of available tANCHOR/FlexTEC vectors and the range of pre-determined Restriction Sites in the basic BioDesign of pFlexMAM.

To learn more about ATG:biosynthetics’ Combined pFlexMAM Protein display system – tANCHOR, please click on the brochure below:

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About ATG: biosynthetics

Advanced Technical Genetomics: biosynthetics GmbH (ATG) is a German-based company that specializes in analytical and functional comparative genomics, molecular biology, immunology and synthetic biology services, systems and applications.

Proper gene functions are the predominant focus of ATG’s activities in the bioinformatics of constructional biology, creating maximum functional impact in design and optimization for its customers.

Located in Merzhausen near Freiburg, ATG offers a complete range of multi-gene and multi-peptide expression products and services for synthetic and molecular biology as well as allied research and development areas.

ATG:biosynthetics possesses particular strengths in designing tailor-made solutions (e.g., gene cluster and complete pathway designs) that harness its technologies and services in related product areas that include gene synthesis, bioinformatics, molecular in silico biodesign, epitope mapping and pathway engineering. These provide clients with individual products and services or integrated solutions that offer a complete product & service package for peptide/gene expression and synthetic biology applications.

ATG seeks partnerships and outsourcing projects that aim at developing and marketing high-potential applications, e.g. creating high efficiency DNA- and protein-based vaccines.

Resources

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Supplier Information
Supplier: ATG:biosynthetics GmbH
Address: Weberstrasse 40, 79249 Merzhausen, Germany
Tel: +49 (0) 761 888 9424
Fax: +49 761 888 9425
Website: www.atg-biosynthetics.com/