By enGenes Biotech…
White Paper – Advancing Biotechnology with enGenes -eXpress™
Advanced biotechnological solutions developer enGenes Biotech has released a new White Paper discussing industrial applications for its ultra-efficient proprietary -eXpress™ platform for recombinant protein expression and secretion.
The paper shows how enGenes -eXpress™ can boost efficiency, quality, and scalability in recombinant protein production, offering solutions to common challenges like low yields, inclusion bodies, and downstream purification complexity. These benefits make it a unique tool for both research and industrial-scale protein production, particularly in pharmaceutical and biotechnology applications.
Decoupling advantages
The article first shows how -eXpress™ decouples bacterial growth from protein production by using T7 RNA polymerase inhibitor, Gp2. This shifts cellular metabolism to recombinant protein synthesis to unlock much higher yields, along with enhanced protein quality. Combining -eXpress™ with enGenes -eXtra™, technology that enables extracellular protein secretion, simplifies purification processes to further and reduce DSP downstream processing costs.
Further advantages include use of L-arabinose tuning of protein production rates, optimizing both cell health and protein quality, making it particularly valuable for complex proteins requiring specific folding conditions, and crucially simplified upscaling to industrial production, with processes developed at liter scale successfully scaled up to 17m3.
Advanced applications
The paper then reviews a range of potential applications, including biopharma production of therapeutic proteins and enzymes with improved solubility and bioactivity, industrial biocatalysis for complex molecule synthesis and specialty chemical production, and in R&D.
The final section reviews a range of case studies, with performance data, of various enGenes -eXpress™ real-life applications. These include:
- A glycosyltransferase scenario in bio-sugar production
- Use of enhanced outer membrane permeability to facilitate secretion of the recombinant protein into the supernatant while avoiding cell disintegration
- Use of VVH antibody (nanobody) fragments secreted into the supernatant to enhance large-scale production in standard fed-batch processes
- Combining enGenes -eXpress™/-eXtra™ to boost staphylococcal Protein A production by increasing coli outer membrane permeability.
The White Paper provides greater insight into the various ways in which enGenes -eXpress™ technology breaks new ground in recombinant protein production, setting new standards in yield, quality, and scalability.