Zyppy™ Plasmid Miniprep Kit

products-servicesNordic BioSite AB

December 1st 2014

Overview

In Scandinavia, Nordic BioSite’ can supply the Zyppy™ Plasmid Miniprep Kit from Zymo Research in the USA.

Zyppy’s pellet-free procedure eliminates the need for conventional cell pelleting and resuspension steps to produce the fastest, easiest miniprep solution on the market for purifying transfection quality plasmid DNA.

Produced DNA quality is appropriate for cloning, sequencing, and transfection.

Features

The Zyppy™ Plasmid Miniprep Kit features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures.

The procedure is simple: add the uniquely formulated 7X Lysis Buffer directly to bacterial or E.coli culture, neutralize, and then purify and elute using the provided Fast-Spin column technology. Additionally, the innovative colored buffers included in the kit permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

Zyppy™ Plasmid Miniprep Kit provides the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.

Products

Zyppy™ Plasmid Miniprep Kits are available* in the following variants.

Catalog No.	Size
D4036	50 Preps
D4019	100 Preps
D4020	400 Preps
D4037	800 Preps

*Contact distributor for supply details

Specifications

Format	Spin Column
Processing Time	Eight minutes
DNA Purity	Plasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A (260/280) index is ≥1.8.
	≥ 30 µl
Plasmid DNA Size	Up to 25 kb
Plasmid DNA Yield	Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.
Plasmid DNA Yield	Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.
Procedure	Performed at room temperature (15-30°C) using a centrifuge and/or a vacuum manifold.

Citations

Zyppy™ Plasmid MiniPrep isolates high quality plasmids for phylogenetic analysis to study methanogen diversity in animal hosts.
Ufnar, J.A. et al., (2007). Development of a swine-specific fecal pollution marker based on host differences in methanogen mcrA genes. Appl. Environ. Microbiolo., 73(16), pp. 5209-5217.
Zyppy™ Plasmid MiniPrep kit used in extraction of pure plasmid DNA in a university research-based molecular genetics laboratory course.
Brame et al. (2008) A molecular genetics laboratory course applying bioinformatics and cell biology in the context of original research. CBE Life Sci Educ. 7(4):pp. 410-421.
Isolating high-quality plasmid DNA from E. coli using Zyppy™ Plasmid Miniprep Kit to generate ~60% increase in titers of resveratrol.
Bhan et al. (2013) Redirecting carbon flux into malonyl-CoA to improve resveratrol titers: Proof of concept for genetic interventions predicted by OptForce computational framework. Chemical Engineering Science, Vol. 103, pp. 109–114

Testimonials

“Performed side-by-side preparations of single culture split into two, using the Zyppy and a leading competitor’s miniprep kit. The Zyppy kit saved me ~25 minutes and returned plasmid of better 260/280.”
Greg G. (North Carolina State University)
“The kit gives a very good yield of plasmid DNA of up to 12 kb in size. I like the fact that procedure can be modified for purification of plasmid DNA from up to 3 ml of bacterial culture.”
Vladimir B (Touro University Nevada)
“The quality of the DNA is very good. Sequencing reads are generally beautiful.”
Magdalena B. (University of Massachusetts, Amherst)

Resources

Click on Zyppy™ Plasmid Miniprep Kit for more information.
Click on Nordic Biosite to contact the company directly.

Supplier Information

Supplier: Nordic Biosite
Address: Propellervägen 4A, 183 62 Täby, Sweden
Tel: +46 (0)8 544 433 40
Fax: +46 (0)8 756 94 90
Website: www.nordicbiosite.com