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Nordic BioSite range of ELISA kits
Nordic BioSite offers a wide and diverse range of ELISA kits and separate components.
ELISA (Enzyme Linked Immuno-Sorbent Assay) is a highly sensitive assay, designed for the in-vitro quantitative determination of a multitude of soluble analytes such as e.g. cytokines, CD antigens, apoptosis markers and growth factors.
BioSite is currently offering a 20% discount on all of its own ELISA kits.
BioSite ELISA assays can be applied to samples from as diverse sources, such as serum, plasma, tissues, cell culture supernatants and other body fluids.
The most commonly used Sandwich ELISA is based on a capture antibody being coated onto a plate surface, allowing the relevant antigen in the sample to bind, and then being detected by a second labeled antibody layer.
A Competitive ELISA plate has the antigen bound the surface, and utilizes only one layer of antibody.
Quantification is made by reading absorption in a spectrophotometer.
A full ELISA plate is good for analysis of approx. 40 samples run in duplicates. Most plates are in a strip-well format, allowing for the use of as few as eight wells at a time.
A typical sandwich ELISA kit contains:
- Precoated plate
- Standard – lyophilized
- Detection antibody – biotinylated
- HRP- streptavidin
- TMB substrate
- Stop solution
- Dilution buffers
- Plate sealer
BioSite’s ELISA range includes a very extensive supply of assays for several species, as well as an abundance of biomarkers, including IL-6, IL-8, IL-10, EGF, VEGF and various cytokines. All of these are presented in the latest brochure, which can be downloaded from the link below (see Resources).
The BioSiteHisto laboratory offers modern, tailored ELISA service to meet specific customer needs.
Expert BioSiteHisto personnel can deliver accurate and reliable ELISA results across an enormous range of permutations of multi-well assays and detection techniques.
BioSiteHisto can also offer customized assays for different sample types or samples from different species.
The BioSiteHisto ELISA service portfolio includes:
- Fluorescence assays
- Luminometric assays
- Colorimetric assays
- Enzymatic activity analyses (e.g. catalase, caspase, peroxidase assays)
- Quantification of biomolecules (e.g. ethanol, glucose, glutathione assays)
- Cellular function analyses (e.g. apoptosis, hypoxia, viability, stress assays)