By Nordic BioSite…
BRAF V600E Rabbit Monoclonal Antibody
Overview
Nordic BioSite has now added BRAF V600E Rabbit Monoclonal Antibody to its portfolio of advanced oncology products, sourced from its new supplier, RevMab Biosciences of California.
Rabbit-derived BRAF V600E providesa ready-to-use IVD IHC monoclonal antibody that eanables researchers to evaluate BRAF V600E mutation expressions with clinical confidence.
Nordic BioSite can now supply this product direct to laboratory customers in the Scandinavian countries (Norway, Sweden, Finland, Denmark and Iceland).
Product Details
From the RAF family of protein kinases, Serine/threonine-protein kinase B-raf (BRAF) constitutes one of the most frequently mutated genes in human tumors.
The RAS/MAPK pathway (Ras-Raf-MEK-ERK signaling cascade) is involved in the regulation of cell proliferation, differentiation, and survival in response to extracellular signaling.
BRAF V600E mutation accounts for the vast majority of BRAF alterations, changing the conformation of the activation segment leading to consecutive phosphorylation of downstream targets.
A mutation at nucleotide 1,799 results in the substitution of valine with glutamic acid at amino acid 600. BRAF gene mutations are found in approximately seven per cent of cancers, while BRAF V600E accounts for more than 90 per cent of these mutations.
BRAF V600E mutations have been detected in a wide range of cancers including melanomas, thyroid and colorectal carcinomas and borderline ovarian cancer.
Citations:
Davies, H, Bignell, G.R., Cox, C., Stephens,P., Edkins, S., Clegg, S., et al. (2002). Mutations of the BRAF gene in human cancer. Nature 417, pp. 949–954.
Sharma, S. (2010) BRAF Mutation Testing in Colorectal Cancer. Arch Pathol Lab Med.; 134: pp. 1225-1228.
Bullock, M., O’Neill, C., Chou, A., et al. (2012). Utilization of a MAB for BRAF V600E detection in papillary thyroid carcinoma. Endocrine-Related Cancer. 19: pp. 779-784.
Pakneshan, S., Salajegheh, A., Smith, R.A., et al. (2013) Clinicopathological relevance of BRAF mutations in human cancer. Pathology. Jun; 45(5): pp. 346-356.
Affloter, K, Samowitz, W., Tripp, S. et al. (2013). BRAF V600E Mutation detection by immunohistochemistry in colorectal carcinoma. Genes, Chromosomes & Cancer. Aug; 52(8):pp. 748-52
Specifications
| Product Name | BRAF V600E Rabbit Monoclonal Antibody |
| Description | Rabbit monoclonal to BRAF V600E Mutant |
| Clone Name | RM8 |
| Specificity | Antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF |
| Immunogen | Peptide corresponding to BRAF V600E mutant |
| Application | Immunohistochemistry, Immunocytochemistry, Western Blot |
| Species Reactivity | Human |
| Conjugate | None |
| Intended Use | Research Use Only |
| Form | Liquid |
| Storage Instructions | Store at 2-8°C |
| Stability | Two years |
| Storage Buffer | PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100ug |
| Concentration | 1 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | IHC, ICC: 2 – 10 ug/mL;WB: 0.5 – 2 ug/mL |
Resources
Click on BRAF V600E Rabbit Monoclonal Antibody for more information.
Click on Nordic Biosite to contact the company directly.
Supplier Information
Supplier: Nordic Biosite
Address: Propellervägen 4A, 183 62 Täby, Sweden
Tel: +46 (0)8 544 433 40
Fax: +46 (0)8 756 94 90
Website: www.nordicbiosite.com
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