Optibodies™: optimized monoclonal antibodies for IHC


Optibodies™: optimized monoclonal antibodies for IHC


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Products & Services | Nordic Biosite AB

Nordic BioSite’s GLP-compliant histology laboratory has developed a range of Optibodies™: monoclonal antibodies that are optimized for immunohistochemistry (IHC).

Optibodies overcome a basic problem for pharma and biotech companies – that while high-affinity monoclonal antibodies play a vital role in many different kinds of research, large-quantity production of IHC-optimized antibodies can be time consuming and expensive.

The tailored BioSiteHisto Laboratory facilities and experienced researchers are ideal for production, purification, and modification of specialized monoclonal antibodies, allowing generation of high quality and target specific antibodies.

Antibody optimization

For BioSiteHisto, antibody optimization is a process in which pretreatment methods, antibody dilution, incubation times, blocking and detection conditions are fine tuned for each antibody under controlled laboratory conditions, measuring values of specificity, sensitivity and stability.

A key success factor is to select correct tissues to express antigens that are appropriately fixed and processed for the specified primary antibody. This is complex because even weakly expressing tissues are relevant, since they offer important information about sensitivity, specificity and signal to noise ratio.

Optimization of antibodies should always be performed with more than one antibody against the same tissue antigen to get results for comparison. Different pretreatment methods are used to fine tune antibody concentration and incubation times. This also helps avoiding one of the biggest challenges in successful optimization, the variations in staining results produced within a laboratory or between different laboratories.

Optibodies

BioSiteHisto-developed Optibodies™ are a series of mouse monoclonal antibodies, specified for the most clinically and diagnostically important markers.

Optibodies are fine-tuned to current clinical needs of today`s laboratory and are also tested against NordiQC quality control standards for control tissues and staining criteria.

The key criterion for each Optibody™ is specificity and high affinity towards its target epitopes. The antibody is also tailored to be flexible and reliable in use, with high consistency between lots.

Additional antibody services

BioSiteHisto can optimize antibodies against human soft and hard tissues as well as rodents, xenograft tissues and other animal models, including rat, rabbit, chicken, goats and sheep.

As well as offering a wide range of ‘off the shelf’ monoclonal antibodies, BioSiteHisto can take on outsourced optimization of customer-specific antibodies, working from supplied slides and tissue sections.

Optibody range

Nordic BioSite’s range of IHC optimized mouse monoclonal Optibodies includes:

Name Aliases Species reactivity Format Sizes
AMACR RACE, alpha-methylacyl-CoA racemase, Human, mouse Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
Androgen receptor KD, AIS, TFM, DHTR, SBMA, HYSP1, NR3C4, SMAX1, HUMARA, AR human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
Bcl2 human Optibodies 100 μl, 1 ml, 7 ml
CD10 Calla, CD-10 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
CD14 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
CD3 T3Z, CD3H, CD3Q, CD3Z, TCRZ, CD3-ZETA, CD247 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
CD31 Platelet endothelial cell adhesion molecule 1 , PECAM1 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
CD326 EPCAM, EP-CAM, ESA, KSA, M4S1, MK-1, DIAR5, EGP-2,EGP40, KS1/4, MIC18, TROP1, TACSTD1 human Concentrate or ready-to-use Optibodies 1 ml, 7 ml
CD38 T10 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
CEA Carcino Embryonic Antigen; CEA; CD66e; DKFZp781M2392; CEACAM5, human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
CK17 CK17, PC, K17, PC2, PCHC1, KRT17 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
CK18 CK-LMW, CYK18, KRT18 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
CK19 K19, K1CS, MGC15366, KRT19 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
CK20 KRT20, Cytokeratin-20, Cytokeratin 20, K20, Keratin 20, Keratin-20, KRT21, CK-20, Protein IT human Optibodies 100 μl
CK5 CK-HMW, DD, EBS2, KRT5A, KRT5, Keratin 5, Cytokeratin5 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
Desmin DES human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
ECAD UVO; CDHE; LCAM; Arc-1; CD324; CDH1 Human, canine, monkey, ovine Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
Endoglin CD105; ENG, END, ORW, HHT1, ORW1, FLJ41744 human Concentrate, Optibodies 100 μl, 1 ml, 7 ml
GCG GLP1; GLP2; GRPP human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
HER2 NEU, NGL, TKR1, CD340, HER-2, HER-2/neu, ERBB2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
Ki67 KIA, Ki-67, MKI67 human, canine Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
MBP MGC99675 human, rat Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
Melan A human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
MLH1 FCC2, COCA2, HNPCC, hMLH1, HNPCC2, MGC5172 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
Napsin A human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
p53 LFS1, TRP53, FLJ92943, TP53 human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
p63a P51B; KET; p63α; p63 alpha, human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
Pan Cytokeratin CK, Keratin, pan CK human, canine Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
PAX5 BSAP human, canine Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
SMA AAT6; ACTSA; ACTA2; Actin alpha 2, smooth muscle, aorta, major constituent of thin filament Human, monkey Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
SYP MRXSYP, SYP, Synaptophysin (p38) human, rat Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml
Vimentin FLJ36605, VIM human Concentrate or ready-to-use Optibodies 100 μl, 1 ml, 7 ml

 

Resources

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Optimized monoclonal antibodies for IHC

Optimized monoclonal antibodies for IHC


Supplier Information
Supplier: Nordic Biosite
Address: Propellervägen 4A, 183 62 Täby, Sweden
Tel: +46 (0)8 544 433 40
Fax: +46 (0)8 756 94 90
Website: www.nordicbiosite.com


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