Trenzyme Products & Services

FreezeFect for Transfection Experiments of Mammalian Cells

Product – Trenzyme GmbH
FreezeFect for Transfection Experiments of Mammalian Cells

Trenzyme GmbH Logo

Contact Supplier: Trenzyme GmbH
Supplier Product: FreezeFect for Transfection Experiments of Mammalian Cells
Address: Byk-Gulden-Str 2, D-78467 Konstanz, Germany
Tel: +49 (0) 75 31 1 22 90 0
Fax: +49 (0) 75 31 1 22 90 11
Website: www.trenzyme.com

FreezeFect for Transfection Experiments of Mammalian Cells

FreezeFect

FreezeFect cells mean you’ll never have to delay transfection experiments because you forgot to thaw your CHO, HEK or HeLa cells or stay late at the lab waiting for freshly thawed cells to adapt to the culture before being able to efficiently transfer them.

FreezeFect cells are transfection competent cells ready-to-use straight out of the cryogenic storage, making the transfect of mammalian cells as convenient as with E. coli. FreezeFect cells are generated with a proprietary Trenzyme technology that was licensed to Lonza in 2011.

This technology is also available as a Nucleofector™ PLUS supplement, which can be used in conjunction with all existing Nucleofector™ kits. No matter the kit and Nucleofector™ device, simply replace the standard supplement (delivered with the regular Nucleofector™ kit) with the appropriate Nucleofector™ PLUS supplement.
FreezeFect

Key benefits:
• Ready to use cryopreserved transfection competent cells
• Eliminates variations caused by the cell culture process
• Every batch is quality certified
• Excellent transfection efficiency and reproducibility over a period of more than one year
• Works with any Nucleofector™ device

Please contact us directly – Within 24 hours we provide you with a sample of FreezeFect for transfection experiments of mammalian cells and any additional information you require.

Fixed Price Protein Manufacturing (FPPM) – Mammalian

Product – Trenzyme GmbH
Fixed Price Protein Manufacturing (FPPM) – Mammalian

Trenzyme GmbH Logo

Contact Supplier: Trenzyme GmbH
Supplier Product: Fixed Price Protein Manufacturing (FPPM) – Mammalian
Address: Byk-Gulden-Str 2, D-78467 Konstanz, Germany
Tel: +49 (0) 75 31 1 22 90 0
Fax: +49 (0) 75 31 1 22 90 11
Website: www.trenzyme.com

Fixed Price Protein Manufacturing (FPPM) – Mammalian

Trenzyme offers guaranteed fixed-price protein manufacturing (FPPM). Protein production is a very costly and time consuming process. Trenzyme can help you free up your resources by outsourcing your protein production.

Our FPPM includes the generation of an expression plasmid, recombinant target protein expression in HEK293 cells and target protein purification. The only thing that we require from you is the cDNA of the target sequence. After receiving the DNA it will take 20 working days to shipment.

Significant Advantages for outsourcing:
• Pay only when we succeed
• No hidden, extra or variable costs
• Complete transparency
• Free feasibility analysis within 24 hours
• Shipped within 20 business days

Product Information:
• 3 mg recombinant protein expressed in HEK293 cells
• Greater than >75 % purity as judged by densitometry of Coomassie-stained gels
• Your choice of N- or C-terminal 6xHis tag
• Purified protein will be delivered overnight on “blue ice” (+1 to 4 °C)
• Detailed specification sheet including QC data
• Shipped within 20 business days from the day we receive the cDNA from you
• Eventual additionally produced protein can be purchased for € 390 per milligram

Cost:
Trenzyme provides 3 mg in HEK293 expressed protein and guaranteed in 20 business days at competitive price of € 3.990.

Please contact us directly – Within 24 hours we provide you with a fixed price protein manufacturing – mammalian quote and information regarding the feasibility of your project.

Fixed Price Protein Manufacturing (FPPM) – E. coli

Product – Trenzyme GmbH
Fixed Price Protein Manufacturing (FPPM) – E. coli

Trenzyme GmbH Logo

Contact Supplier: Trenzyme GmbH
Supplier Product: Fixed Price Protein Manufacturing (FPPM) – E. coli
Address: Byk-Gulden-Str 2, D-78467 Konstanz, Germany
Tel: +49 (0) 75 31 1 22 90 0
Fax: +49 (0) 75 31 1 22 90 11
Website: www.trenzyme.com

Fixed Price Protein Manufacturing (FPPM) – E. coli

Trenzyme offers guaranteed fixed-price protein manufacturing (FPPM). Protein production is a very costly and time consuming process. Trenzyme can help you free up your resources by outsourcing your protein production.

Our FPPM includes the generation of an expression plasmid, recombinant target protein expression in E. coli and target protein purification. The only thing that we require from you is the cDNA of the target sequence. After receiving the DNA it will take 20 working days to shipment.

Significant Advantages for Outsourcing:
• Pay only when we succeed
• No hidden, extra or variable costs
• Complete transparency
• Free feasibility analysis within 24 hours
• Shipped within 20 business days

Protein Production Information:
• 3 mg recombinant protein expressed in E. coli
• Greater than >75 % purity as judged by densitometry of Coomassie-stained gels
• Your choice of N- or C-terminal 6xHis tag
• Purified protein will be delivered overnight on “blue ice” (+1 to 4 °C)
• Detailed specification sheet including QC data
• Shipped within 20 business days from the day we receive the cDNA from you
• Eventual additionally produced protein can be purchased for € 330 per milligram

Cost:
Trenzyme provides 3 mg in E. coli expressed protein and guaranteed in 20 business days at competitive price of € 2.990.

Please contact us directly – Within 24 hours we provide you with a fixed price protein manufacturing – E. coli quote and information regarding the feasibility of your project.

Recombinant Target Expression and RNAi-Mediated Target Knockdown

Product – Trenzyme GmbH
Recombinant Target Expression and RNAi-Mediated Target Knockdown

Recombinant Target Expression and RNAi-Mediated Target Knockdown

Contact Supplier: Trenzyme GmbH
Supplier Product: Recombinant Target Expression and RNAi-Mediated Target Knockdown
Address: Byk-Gulden-Str 2, D-78467 Konstanz, Germany
Tel: +49 (0) 75 31 1 22 90 0
Fax: +49 (0) 75 31 1 22 90 11
Website: www.trenzyme.com

Recombinant Target Expression and RNAi-Mediated Target Knockdown

Trenzyme’s technology platforms for recombinant target expression and RNAi-mediated target knockdown regulation in mammalian cells, the ExoIN and EndoOUT systems, have proven to be extremely valuable tools to rapidly generate highly individualised cell lines. Due to Trenzyme’s expertise, unique experience and technology, assay cell lines and protein production cells lines can be functionally validated very early on in the stable cell line development process.

Recombinant Target Expression and RNAi-Mediated Target Knockdown

Some key features of the systems are:

• Stringent coupling of selectable markers with recombinant target expression and RNAi-mediated target knockdown
• No requirement for clonal cell lines due to extreme homogeneity within the whole population
• Three day turn around for homogenous expression or knockdown
• Fast feasibility turn around within a week
• Stable and homogenous cell line availability within two weeks
• Tuneable expression (ExoIN)
• Maximum knockdown from single copy integration (EndoOUT)
• ExoIN and EndoOUT are compatible with any cell line
• ExoIN technology works with any target protein (secreted, membrane, nuclear and cytoplasmic)
• Available as inducible or constitutive systems

Trenzyme is a leading expert in recombinant target expression and RNAi-mediated target knockdown regulation in mammalian cells using the latest technology by Trenzyme – ExoIN and EndoOUT. Trenzyme’s technology has a highly successful track record lead by experts in the field to efficiently and successfully create custom cell lines for our clients.

Optimising and Custom Recombinant Protein Expression

Product – Trenzyme GmbH
Optimising and Custom Recombinant Protein Expression

Optimising and Custom Recombinant Protein Expression

Contact Supplier: Trenzyme GmbH
Supplier Product: Custom Recombinant Protein Expression
Address: Byk-Gulden-Str 2, D-78467 Konstanz, Germany
Tel: +49 (0) 75 31 1 22 90 0
Fax: +49 (0) 75 31 1 22 90 11
Website: www.trenzyme.com

Optimising and Custom Recombinant Protein Expression

Trenzyme is an expert in optimising and custom recombinant protein expression. Due to Trenzyme’s modular cross-organism expression technology platform we can screen and optimise the expression of a given target protein in parallel, exploiting the individual strengths of the four most acknowledged host systems: E. coli, yeast, insect (Baculo-system) and mammalian cell. As a great benefit for our customers Trenzyme can deliver a fast feasibility answer, streamlining the project development and therefore ensuring a substantial time saving for optimising and optimising-and-custom-recombinant-protein-expression custom recombinant protein expression.

By using the latest technology and equipment, Trenzyme can realise custom recombinant protein expression and purification according to the needs of the customer. A vital network of collaboration partners enriches the sophisticated in-house possibilities, which also allows the development of complete upstream and downstream processes including exceptional analytical capabilities.

Custom Recombinant Protein Expression

If our clients are interested in the synthesis of a protein we can offer a custom protein expression solution to meet the targets of our clients, and we can adapt and customize to the needs of the client and the recombinant protein. If the timeline is extremely short as it happens to be for many clients and the requirement is for small quantities of the protein we use our expertise to select an appropriate system to deliver your recombinant protein expression. Trenzyne also offers full up scaling and our capacity and in-house expertise ensures we can deliver custom recombinant protein expression solutions beginning with test expressions in millilitres to high cell density fermentation in a bioreactor with full computer process control.

Trenzyme’s technology platforms for custom recombinant protein expression or RNAi-mediated down-regulation in mammalian cells, the ExoIN and EndoOUT systems, are proven to be extremely valuable tools to rapidly generate highly individualised cell lines.

Stable Mammalian Cell Line Development

Product – Trenzyme GmbH
Stable Mammalian Cell Line Development

Stable Mammalian Cell Line Development

Contact Supplier: Trenzyme GmbH
Supplier Product: Mammalian Cell Line Development
Address: Byk-Gulden-Str 2, D-78467 Konstanz, Germany
Tel: +49 (0) 75 31 1 22 90 0
Fax: +49 (0) 75 31 1 22 90 11
Website: www.trenzyme.com

Stable Mammalian Cell Line Development

Stable mammalian cell line development and mammalian cell culture is completely necessary for the successful development of assay cell lines which resemble human physiology. Cell line development can be time consuming in particular with protein expression however may be necessary in regards to native-host conformation or specific post translational modifications, which need to be present to study a particular protein function. Trenzyme uses highly customized cell lines to obtain high quality transferable results in drug screens. Trenzyme is an expert in stable mammalian cell line development and mammalian cell culture and our customers can always rely on our speciality in cell biology to provide new and innovative concepts in the area of stable mammalian cell line development providing functional cellular assays and protein production.

Mammalian Cell Line Development

Trenzyme’s expertise, resources and unique technology ensures assay cell lines and protein production cell lines can achieve early validation within the mammalian cell line development process and other cell line development processes ensuring optimum results for our clients therefore saving them time and money in their cell line development processes. Trenzyme offers expertise advice and can consult with you to establish a productive and successful cell culture strategy to reach our clients cell culture targets and goals including but not limited to stable mammalian cell line development.