Nordic BioSite supplies ZymoPURE Maxiprep ‘18 minute Plasmid Isolation’ kit

products-servicesNordic BioSite AB
July 30th 2015

 

Overview

In Scandinavia, Nordic BioSite’ can supply the ZymoPURE™ Plasmid Maxiprep Kit from Zymo Research in the USA.

ZymoPURE provides the fastest and simplest method so far developed for extraction of ultra-pure endotoxin-free plasmid DNA from E.coli.

The plasmid isolation kit allows researcher to routinely recover ≥ 1 µg/µl plasmid DNA directly from a microcentrifuge column that is ideal for transfection and other sensitive downstream applications. The eluted transfection quality plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.

The Maxiprep kit features a spin column-based method, allowing ultra-rapid purification of up to 1.2 mg of high-quality plasmid DNA in less than 18 minutes.

The company has posted a video that shows a researcher performing a plasmid DNA isolation in 15 minutes and 43 sec.

Features

ZymoPURE™ technology uses a modified alkaline lysis method. Its novel binding chemistry yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. This unique design allows the binding step to be performed using a vacuum or tabletop centrifuge.

Zymo has optimized the wash regimen to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. This produces plasmid DNA that is suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.

The ZymoPURE™ Plasmid Maxiprep also features convenient colored buffers to assist error-free visualization and identification of complete bacterial cell lysis and neutralization.

The kit includes syringe filters for rapid clearing of the lysate.

Products

ZymoPURE™ Plasmid Miniprep Kits are available* in the following variants.

Specifications Testimonials“Performed side-by-side preparations of single culture split into two, using the ZymoPUREand a leading competitor’s miniprep kit. The ZymoPUREkit saved me ~25 minutes and returned plasmid of better 260/280.”
Greg G. (North Carolina State University)“The kit gives a very good yield of plasmid DNA of up to 12 kb in size. I like the fact that procedure can be modified for purification of plasmid DNA from up to 3 ml of bacterial culture.”
Vladimir B (Touro University Nevada)“The quality of the DNA is very good. Sequencing reads are generally beautiful.”
Magdalena B. (University of Massachusetts, Amherst)“I used plasmid for transfection and obtained a very high expression of recombinant protein. The plasmid is well tolerated by insect cell cultures and transfected efficiently, comparable to caesium chloride gradient prep.”
M. A., University of Paris VI“DNA obtained from using the ZymoPURE kit was ten times the amount of DNA obtained from using the QIAGEN kit.”
J. M., Vanderbilt University“Great product! Very convenient and fool proof. I would feel comfortable to let interns or people with little experience using this kit and still getting great results.”
X. L., University of Alabama at Birmingham“I was very satisfied with this product. It produced high DNA concentration eluting in 400 µl using the above mentioned cell line, which would generally only be obtainable with other kits eluting in much smaller volumes. This product is also miles ahead of other general Maxiprep kits in terms of time required for plasmid extraction.”
J. P., University of Pretoria“We were impressed by the high yield of plasmid DNA from this kit! From a 150 mL culture, I was able to isolate 0.912 mg DNA, in the same amount of time (and effort) I could get about half as much DNA from the comparable QIAGEN kit.”
C. S., Abbott LaboratoriesResourcesClick on Nordic BioSite supplies ZymoPURE Maxiprep ‘18 minute Plasmid Isolation’ kit for more information.
Click on Nordic Biosite to contact the company directly.Supplier Information
Supplier: Nordic Biosite
Address: Propellervägen 4A, 183 62 Täby, Sweden
Tel: +46 (0)8 544 433 40
Fax: +46 (0)8 756 94 90
Website: www.nordicbiosite.com

Catalog NO Size
131 10 Preps
131 20 Preps
Processing Time 18 min
Equipment Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge.
DNA Purity Eluted DNA is ultrapure and well suited for transfection, sequencing, cloning, plasmid mediated gene silencing, in vitro transcription, and other sensitive applications.
Plasmid DNA Size Up to 25 kb
Plasmid DNA Yield Up to 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)
Recovery Volume ≥ 200 µl of ZymoPURE™ Elution Buffer or DNase free water